Celluclast 2L, Novozyme 188

Optimization of temperature and enzyme meanness in the enzymatic saccharification of steam-pretreated willow tree tree Robert Eklund, Mats Galbe and Guido Zacchi Chemical locomotive room I, University of Lund, Lund, Sweden The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to accommodate the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 come down match to the results of earlier optimization studies of the steam-pretreatment conditions for lavishly glucose regainings. The concentrations of devil technical enzymes, Celluclast 2L and Novozyme 188, were vary in the range 0-20% (based on oven-dry subst vagabond). A maximum in the conversion o f cellulose to glucose was obtained using a sort of 15% Celluclast and 3% Novozyme, interchangeable to an activity of 11.4 FPU g-~ substrate. higher(prenominal) enzyme concentrations did not cast up the sign hydrolysis rate nor be active the utmost glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects some(prenominal) the initial hydrolysis rate and the terminal glucose yield. The highest glucose yield was obtained at 40°C.

The yield decreased with change magnitude temperature, collect to increased enzyme deactivation at higher temperatures, speckle the initial reaction rate increased with increasing temperatures. Keywords: Enzymes; saccharification; steam-pretreatment; willow; hydrolysis; temperature Introduction The make up of roughly material and enzymes are the both major follow components in the bioconversion of lignocellulosic material to ethanol. =In a previous study,2 the cost of enzymes were prove to constitute about 80% of the total hydrolysis cost. It is gum olibanum necessary to optimize the enzymatic hydrolysis to obtain high yields at low enzyme consumption. In two previous studies, 3,4 steam pretreatment was shown to be a very efficient method of making the huffy material, willow, accessible for enzymatic saccharification. Glucose...If you call for to get a comprehensive essay, order it on our website: Ordercustompaper.com

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